Genotyping of Avian Corona Virus from Broilers Infection in Babylon Governorate, Middle Iraq.

Abstract

This study aimed to track the serotypes of infectious bronchitis virus in poultry Meat farms in Babylon province in the middle of Iraq that are suffering from respiratory symptoms.Fifty poultry flocks were included showing respiratory signs, a rapid examination was performed using the IBV Rapid kit, 13 flocks showed a positive result for the rapid examination, Samples were taken randomly from the kidney and the trachea, from which the RNA was extracted, then converted to complementary DNA cDNA, this last product was entered into a real-time PCR with XCE2+ and XCE2- primers, 7 samples showed a positive result.Special primersMCE1+, BCE1+ and DCE1+ for known pathogenic IBV strains Massachusetts, D274, & 793/B,respectively, were used in a nested PCR, that gave positive results for all three isolates. The flocks were vaccinated with each of the Massachusetts and 793/B strains. Nevertheless, an infection appeared that may be due to the failure of the vaccine, or the immunosuppression. The flocks did not receive the D274 vaccine, and here the issue of introducing them to the vaccination programs must be studied. The isolates that appeared represented the primers, and this does not deny the existence of other isolates, and to prove this, it is recommended to conduct genetic sequencing, which is a future work to complete this study.