Genome Sequence Analysis of the Bacillus Cereus Isolated From Soil Sample

Abstract

In Recent decades researches investigated that groups of the Bacillus cereuswill carry genes may have the capacity to cause gastrointestinaland somatic diseases. Despite the fact that most of the diseases caused by B. cereus group pathogens are relatively acute, scientist have identified from food and in the environment to investigate members of the B. cereus group. This current research was aimed to isolate and identify Bacillus species from soil sample. The Bacillus species isolated were identified byusing the classical method. The pure isolate was subjected to morphological tests, biochemical tests and gram staining. The antibiotic sensitivity test was studied using Cefotaxime/ clavulinic acid, Cefazolin, Ampicilin, Doxycycline, Methicilin, Gentamicin, Tobramycin, Rifampicin, Tetracycline and Erythromycin for the isolated bacteria from soil. The results showed high activity of zone of inhibition for Cefotaxime/ clavulinic acid, Doxycycline, GentamicinTobramycin,Tetracycline and Erythromycin, showed moderate zone of inhibition for Rifampicin and Methicilin.The same isolates were further identified by DNAsequencing method;Fragment of 16S rDNA gene was amplified by 27F and 1492R primers.The 16S rDNA gene sequence was used to carry out BLAST with the database of NCBI genbank database.PCR analyseswith DNA from B. cereushave been confirmed the specificity of the PCR assay. Forward and reverse DNA sequencing reaction of PCR amplicon was carried out with forward primer and reverse primers using BDT v3.1 Cycle sequencing kit.The study suggests that some of Bacillus spp from the study area have potential to produceantibiotics that may be used to control microbial growth.