Study The Role of Nigella Sativa Silver Antiparticles on Some Liver Biomarkers and Hepatocyte Comet Assay in Stressed Rats

Abstract

Nigella sativa silver nanoparticles (NS-Ag-NPS) were prepared and characterized for investigation the liver dysfunction induced by H2O2 in rats. forty healthy adult male rats selected and divided randomly into five groups (eight rats per group). The control group (G1) was administrated with tap water for 2 months. The second group (G2) was administrated tap water with H2O2 1% for 2 months. Third group (G3) was IP injected with Nigella sativa seed aqueous extract (60 mg/ Kg/ body weight) and receive tap water with 1% H2O2 for 2 months. The fourth group (G4) was IP injected with NS-Ag NPs (30mg / Kg/ body weight) and receive tap water with 1% H2O2 for 2 months. Finally, the fifth group (G5) was IP injected with NS-Ag NPs (60 mg / Kg/ body weight) and receive tap water with 1% H2O2 for 2 months. At the end of the experiment, rats were anesthetized and blood samples were collected through cardiac puncture procedure, for measurement of serum AST, ALT, and ALP, and liver tissue homogenized for measurement of (GPx) and (MDA) level. The histological changes examination of liver tissue and comet assay technique for DNA fragmentation of hepatocyte was studied. The results showed significant (P<0.05) elevation of AST, ALT, ALP, and MDA in G2, G4, and G5 group as a compared with G1 and G3 group, A significant (P< 0.05) decrease of GPx level in G2, G4, and G5 groups as a compared with G1 and G3 group. A significant (P ˂0.05) elevation of medium % and high % of comet appearance in G2, G4, and G5 groups as a compared with G1 and G3 groups. On the other hand, the percentage of no comet appears significantly (P ˂ 0.05) in G1 and G3 groups. All comet parameters showed a significant (P ˂0.05) elevation in G4 and G5 groups as compared with the control group except in %DNA in Head parameter which showed a significant (P< 0.05) decrease when compared with the G1 group. On the other hand, the G3 group showed no significant differences from the control group. Histological section of the liver was illustrating damage of hepatocyte, mononuclear cell, infiltration of inflammatory cell, vacuolated hepatocytes and focal necrosis area was observed in liver tissue after administration with H2O2, NS-Ag NPs (30mg / Kg/ body weight), and NS-Ag NPs (60mg / Kg/ body weight). Finally, the current study's findings explained the cytotoxic activity of NS-Ag- NPs, and H2O2 at high concentrations, as well as the ameliorative impact of NS and NS-Ag- NPs at low concentrations on certain liver biomarkers.