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A non-probability (Convenience) sample of (100) adolescent patients with major, were included in the present study. The data were collected through the utilization of the developed questionnaire which consists 4 parts: Demographic characteristics, Information regarding Thalassemia, Information regarding Deferoxamine, self injection of Deferoxamine (practice). The study was carried out at A-Zahraa Teach Ling Hospital in AL-Najaf City, 1/12/2018 – 1/4/2019, to assess Knowledge and practice of self administration about injection of Deferoxamine among adolescent thalassemia patients in al-Najef city and to find out the association between Sociodemographic characteristics and Knowledge and practice of self administration about injection of Deferoxamine. The present study findings designated that a. age group (16-18) years more frequency in this study. Most study sample in rural area, and secondary school. The overall assessment of studied domains is intermediate. The study indicates that there is a significant association between demographic data and practice except (gender, occupation of mother, type and number of family, residency). The study indicates that there is a significant association between demographic data and knowledge except gender. The researcher recommends Confirming on thalassemia teenager to have appropriate information about injection of Deferoxamine and major thalassemia care to avoid health problems during life of patient. Educational programs should be emphasized to adolescent thalassemia teenager for increasing their knowledge and interest about injection of Deferoxamine. Further studies should be conduction with a larger sample (national level) including both rural and urban populations.