Main Article Content
P. aeruginosa isolated from water and soil samples and identified by Vitek 2 system. Based on primary screening assays such as (hemolysis, CTAB, oil dispersion, drop collapse, and emulsification index (E24 % ) methods, 5 isolates were chosen for their ability to produce biosurfactants. The identification of 5 isolates were confirmed by 16SrDNA gene. Crud biosurfactant purified by column chromatography and obtained pure rhamnolipids honey colour product. TLC, FTIR, GC-MS, and HPLC were used to characterize the biosurfactants. The biosurfactant was found to be rhamnolipid. Rhamnolipid prevent the adhesion of P. aeruginosa, E. cloacae, S. aureus, P. mirabilis cells and biofilm formation of these bacteria. Rhamnolipid also disrupted the pre-formed biofilms of these bacteria.