Transcriptome Analysis of some resistant genes of Multidrug Resistant Salmonella serovars isolated from Egypt

Abstract

Multidrug resistant Salmonella strains have been considered as the most prevalent food-borne illness worldwide. The widespread contamination of meat and meat by products with Salmonella had given rise to a global clinical concern. A total of 100 samples were collected randomly from different sources. The bacterial isolates were identified by conventional bacteriological identification, followed by biochemical testing on VITEK2C, and then all identified Salmonella isolates were further confirmed by Real-time PCR. Antimicrobial susceptibility phenotypes were determined for forty positive identified salmonella specimens by disc diffusion method; significant resistances to four antibiotics were observed among twelve isolates. The expression of resistant genes was investigated through the extraction of mRNA from resistant isolates and undergone RT-PCR. Molecular analysis identified the presence of the resistance genes blaTEM-1(50%), dfrA1 (50%), tet (B) (41.7%), sul1 (66.7%) in tested isolates. These results indicate that Salmonella isolates under study showing multidrug resistance to some antibiotics through expression of its resistance genes. Our study revealed that, Fresh meat and meat byproducts were contaminated by multidrug resistant Salmonella would be causing the foodborne illnesses.