Association Between HOXB13 Gene Genotypes and Prostate Cancer in Basrah, Iraq

Abstract

Prostate cancer is the sixth most common cancer in the world, the second most common cancer in men in developed countries. Development of prostate cancer as any other cancer could be provoked by accumulation of mutations in various genes: oncogenes, tumor suppressor genes, DNA repair genes, and epigenetic alterations. The mammalian Hoxhomeobox genes are transcription factors that regulate axial regional specialization during embryonic development. The present study including 67 patients and 70healthy control, genomic DNA was extracted from all blood samples (patients and controls) and the HOXB13 gene was amplified using PCR technique and sequencing in order to detect the genetic variation. Three variation sites were detected in exon one of HOX B13 gene, A1098G (rs9900627), G951A (rs8556), C773T (rs199813155). As a result of changing the base A by G in (A1098G) rs9900627, three polymorphisms were detected, the statistical analysis found that AG polymorphism increases the risk of disease about one and a half fold (OR=1.4) while AA and GG were not significant.Our study detected ten genotypes in exon 1, AGC genotype was the wild type while the other nine were novel and recorded in DDBJ as a new genotype in the world RGC (LC483165.1), GGC (LC483166.1), RRC (LC483167.1), ARC (LC483168.1), AAY (LC483170.1), ARY (LC483172.1), AAC (LC483173.1), AGY (LC483171.1) and RGY (LC483169.2).