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The discharge of untreated effluents during the starting of new industries and development of the industries results in water, air and soil pollution. During this period industrial waste effluent considered as a pollution causer because of the disposal of untreated effluent. Water pollution is the major pollution that effects the environment much and one of the sources of this pollution is tannery industrial effluents. Effluents are so toxic that fishes cannot survive in it even for two hours and also effects the drinking water and hence it should be treated effectively before release. Bioremediation is the only way to tackle these xenobiotics and reduce the pollutants which is eco-friendly. Keratinase enzymes and halophilic microorganisms are ubiquitous in nature and usually found in all living organisms. This paper based on the characterization and GCMS analysis of tannery effluent by using halophilic bacterial strain Pseudomonas aeruginosa sthc002 and keratinase enzyme. An experiment were conducted to isolate keratinase producing microbial strain from tannery effluent from Puthalam salt pan, Kanyakumari District, Tamil Nadu. Biochemical tests were done to identify the microorganisms.