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During this study a total of (250) samples were collected from patients suffering from burns and wound infections from three hospitals in Baghdad during the period from (1/9/2019) to (30/12/2019). The isolates were firstly identified by a microscopic examination and their cultural characteristics on different selective media, then finely identified by VITEK-2 system. Results revealed that only 120 isolates (48%) of them were gave a typical morphological characteristic and biochemical test that belong to P. aeruginosa isolates. The molecular study included the detection of virulence genes toxA and exoS in P. aeruginosa isolates by colony PCR technique. Results revealed that toxA and exoS genes were found nearly in all P. aeruginosa isolated from burn and wounds infections, the toxA gene was detected in 27/30(90%) of isolates. Also, exoS gene was found in 26/30 (86.7%) of isolates.