Cytotoxicity Effects of Guava Leaf Extract on Fibroblast Cells- An Invitro Study

Abstract

Root canal infections are polymicrobial in nature and as we know there are a number  of mechanisms involved in eliminating these infectious microorganisms. From access opening to obturation,a constant  used to  eliminate the biofilm is endodontic irrigants. The aim of this study was to evaluate the effect of Sodium hypochlorite and Guava leaf extract on the L929  fibroblast cell line.

Materials and Method

The guava leaf powder was obtained and the extract was prepared. For cell viability assay, cells were seeded onto 96-well plates at a concentration of 5×103 cells/well, followed by the addition of test samples of various concentrations prepared in cell culture media. MTT assay was done which measures the reduction of yellow 3-(4,5dimethiazol-2-yl)- 2, 5-diphenhydramine tetrazolium bromide(MTT) by mitochondrial succinate dehydrogenase. The effect of test samples (25-100μg/ml) on cell growth inhibition will be assessed as percentile viability, where vehicle-treated cells will be taken as 100% viable.

Results

Statistical analysis was done using One-way Anova and a post hoc test. p<0.05 was considered as significant. P<0.001 as compared with negative control. Guava leaf extract at different concentrations are P<0.001 as compared with sodium hypochlorite.

Conclusion

Guava leaf extract had no or less cytotoxic effect based on its concentration. Hence it can be used as an alternative endodontic irrigant.