Endophytic Bacteria as Apotential Agent for Control of Tomato Wilt Caused by Fusariumoxysporum F.Sp Lycopersici
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Abstract
This study was conducted at the college of Agriculture / University of Basrah during 2020/2021 season ,with aim of isolating the endophytic bacteria from internal tissue of healthy tomato plants and their associated weeds and evaluating their efficiency in reducing tomato wilt caused by Fusarium oxysporum f.sp lycopersici . Ten isolates out of eighty-two isolate obtained from tomato plants and some associated weeds showed efficiency in inhibiting the growth of the F.O.L both in well and dual culture technique. Identification of endophytic bacteria by VITEK2 device showed that three of them belong to Pseudomonas aeruginosa , one belong to P.putida while the others belong Staphylococcus lentus ,Enterobacter dissolvens , Alloiococcus otitis and Serratia marcesens. Pot experiment showed that soaking tomato seedling roots with suspension of P.aeruginosa or P.putida led to a reduction of tomato wilt incidence from 100% in control treatment to 41.7% and disease severity from 45.8% to 16.7%,and increased chlorophyll content and polyphenol oxidase and peroxidase activety.