The Difference in Endothelial Progenitor Cells (Epcs Cd34), Clotting Factors, Blood Pressure and Proteinuria among Preeclampsia Model Mice with and without the Administration of Epcs Cd34

Abstract

Background: Preeclampsia is the leading cause of mortality in 15-20% of pregnant women worldwide. EPC is part of a hematopoietic stem cell marker molecule, namely CD34. EPC have a strong relationship with the improvement of endothelial function and blood angiogenesis process. Until now, there are not many investigations regarding the potential of EPC for therapy for preeclampsia, although it is known that in people with preeclampsia the number of EPC has decreased. The use of EPCs as an alternative to endothelial cell regeneration in the future has enormous potential in its use as modern therapies.

Objective: To determine the differences in CD34 EPC counts, platelet aggregation levels, fibrinogen, D-dimer, PAI-1, blood pressure, and proteinuria among preelamptic model pregnant mice with and without administration of EPCs CD34.

Methods: This research method is pure experimental using 3 stages of research analyzed using unpaired t test or Mann Whitney test if the data is not normally distributed. All research data will be analyzed using SPSS version 22 software.

Research result: In this study, the average number of CD34 ECPs in the group given EPCs CD34 was higher (7.334 ± 0.891 vs 7.040 ± 0.473), p value > 0.05. The average platelet aggregation in the group given EPCs CD34 was lower (27.61 ± 8.93 vs 43.56 ± 1.38), p value <0.05.Fibrinogen levels in the group given EPCs CD34 were lower (1.102 ± 0.065 vs 0.944 ± 0.074), p value <0.05.D-Dimer levels in the group given EPCs CD34 were higher (7,778 vs 2,266), p value  <0.05.PAI-1 levels in the group given EPCs CD34 were higher (4.168 ± 0.564 vs 3.565 ± 0.802), p value > 0.05. Systolic and diastolic blood pressures in the group given EPCs CD34 were lower (89.8 ± 8.9 vs 187.0 ± 25.0) and (72.3 ± 10.8 vs 174.7 ± 34.4), p value <0.05.Proteinuria in the group given CD34 EPCs was lower (+4 vs +1) , p value <0.05.

Conclusion: In the preeclamptic pregnant mice group given EPCs CD34: There was no difference in the number of EPCs CD34 & levels of PAI-1, but levels of platelet aggregation, fibrinogen, blood pressure, & proteinuria in the group of preeclamptic pregnant mice that were given EPCs CD34 were lower, while D-dimer levels in the preeclamptic pregnant mice group given EPCs CD34 were higher than those in the preeclamptic pregnant mice group that were not given CD34 EPCs.