Effect of Mannitol and PEG on the Accumulation of Rutin in Callus culture of Ruta Graveolens

Abstract

Rutin, a secondary metabolite contained in Ruta graveolens, has been used in medicine for a variety of applications. Elicitation of mannitol and polyethylene glycol (PEG) for Rutin development enhancement in Ruta graveolens callus culture would be a viable alternative. Cell growth and Rutin production were also affected by mannitol and PEG. The most effective elicitation method was to treat cell cultures with 10 mg/l PEG, that resulting in the high Rutin output (0.70 mg/g DW). PEG elicitation is a useful method for growing Rutin production in Ruta graveolens callus culture.