Characterization and Evaluation of the Antibacterial Profile of ZnO Nps Synthesized by Liv-Pro-08 Ayurvedic Herbal Formulation (AHF)

Abstract

This study was performed to determine the Zinc Oxide nitrate reducing potential of aqueous extract of Liv-Pro-08 polyherbal formulation and characterize the synthesized ZnO NPs and assess their antibacterial activity against pathogenic bacteria such as Staphylococcus aureus Bacillus cereus, Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa. The results showed that the yellow-colored crystalline ZnO NPs were synthesized, and it was preliminarily confirmed by UV analysis by attained a predominant peak at 375 nm. Through Powder X-ray diffraction (PXRD) and Fourier-transform infrared spectroscopy (FT-IR) analyses, the crystalline structure and functional groups exist in the surface of ZnO NPs, and its size was measured as 2 to 5 µm through Scanning Electron Microscope (SEM) analysis, and morphology was found as spherical with the smooth-edged surface. The antibacterial potential of characterized ZnO NPs was assessed, and found significant antibacterial activity against pathogenic bacteria such as S. aureus B. cereus, E. coli, K. pneumonia, and P. aeruginosa with 100 µg concentration of various volumes (25, 50, and 75 µl) of nanoparticles. These results conclude that the aqueous extract of Liv-Pro-08 polyherbal formulation synthesized ZnO NPs might be used as an antibacterial agent against some common pathogenic bacteria.