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The World Health Organization defines the diabetic foot as a “situation of infection, ulceration or also destruction of tissues depths of the feet, associated with abnormalities neurological and varying degrees of vascular disease peripheral in the lower limbs of patients with Diabetes Mellitus. The objective of the work was identify the prevalence of microorganisms that caused infections in diagnosed patients with diabetic foot treated at the Regional Al-Dewaniyah Hospital, Iraq, during the year 2020. Descriptive, cross-sectional and retrospective study. The sampling was non-probabilistic, for convenience, and 115 samples corresponding to 94 patients. Of the 94 patients with diabetic foot, 52% were mens. 25% of infections occurred in patients aged 51 to 60 years. 21 different microorganisms were isolated in the 115 samples. 80% (75) were monomicrobial, and 20% (40) polymicrobial. Among the Gram positives, the most frequently isolated microorganism was the Staphylococcus aureus 19% (22) and Enterococcus spp. 6% (6) and among the Gram negative were the Klebsiella pneumoniae13% (16) and Acinetobacter spp. 12% (14). The results of sensitivity tests antimicrobial agents showed that 100% of the S. aureus strains were resistant to Oxacillin and high resistance of K. pneumoniae strains to Cephalosporins. Acinetobacter Strains spp. were 100% resistant to cephalosporins and Piperacillin. Isolated microorganisms and antimicrobial resistance profile as the same present coincide with the bibliography, and it is very important to implement prevention programs this pathology in order to avoid amputations in this type of patients.