Non Enzymatic Method to Isolation Toxoplasma Gondii from Placental Tissue and Effect on Lipid Peroxidation in Pregnant Women in Al-Ramadi City

Abstract

Background: Toxoplasmosis is known to be one of the most common infections worldwide in humans and warm-blooded animals .One-third of the world’s population is infected with toxoplasmosis. The current study aimed to isolate the toxoplasma gondii parasite from aborted and un aborted placentas for women with toxoplasmosis within AL Ramadi city by non-enzymatic methods to take care of the vitality of the rapidly multiplying phase of the parasite found within the placental tissue. Additionally , the present study aims to detection the effect of parasitic on Lipid Peroxidation by measuring malondialdehyde (MDA) levels within the pellet of solution extracted from the placenta after mashing it to examine the parasite The results of the microscopic examination of the pellet and suspention that extracted from placental solution showed the presence of bradyzoite in most of the diagnosed samples additionally to the emergence of the rapidly multiplying phase tachyzoite of others. The results of Lipid Peroxidation measurement showed increase in MAD levels in infected samples compared to control samples but its non-significant .