In Vivo Study of Probiotic Role in Protection and Prevention of Helicobacter Pylori that Cause Stomach Ulcer in Rats

Abstract

To evaluate the probiotic role in protection and prevention of Helicobacter pylori that cause stomach ulcer. H. pylori were isolated from patients with gastric ulcers. The probiotic was purchased from pharmacy which used in this study. Forty male rats were divided into 4 groups, G1 serve as control negative given only distil water, G2 given only Probiotic for 30 consecutive days, G3 given only H. pylori at 10⁷ CFU/ml, G4 given Probiotic for 30 consecutive days then infected with H. pylori at 10⁷ CFU. The results of invitro study showed susceptibility of H. pylori isolates to Probiotic and Ciprofloxacin, while it presented resistant to other antibiotics used. The histopathological results showed the bacteria appeared to be adherent to the gastric epithelium in G3. The rats developed chronic active gastritis in  G3 closely resembling the H. pylori gastritis observed in humans, while the histopathological G4 showed apparently normal mucosa.

In conclusion, the usage of probiotics for 1 month can decrease the chance of H. pylori infection that causing gastric ulcer.