Preparation and Study of a Novel Thiazole Derivative and Its Biological Efficacy on Male Rats Infected with Echinococcus Granulosus Using Electron Microscope

Abstract

This study included the preparation of a novel thiazole derivative and its spectroscopic diagnosis, as it was prepared from crushing solid iodine with Thiourea well, then adding it with Pregnenolone. The compound was diagnosed using spectroscopic methods that included an IR Spectra, ¹H-NMR spectra and a mass  spectra, and the results were virtually identical to expectations.

       The lethal dose was determined to half 50 (LD₅₀) for the compound after it was dissolved with DMSO, and then the safe therapeutic dose was calculated, which was 0.18 g/ kg.

This compound was dosed orally as a therapeutic dose for a group of male rats, after they were infected with hydatid cysts. To find out the therapeutic efficacy of it on the hydatid cyst, and to compare it with a group that was infected with the same disease and treated with Albendazole, the dosing period lasted 30 days for each of the above groups.

The results of the Field Emission Scanning Electron Microscope (FE-SEM)study of the hydatid cystsof the positive control group showed that the hydatid cyst contains aGerminal Layer (GL) and contains large numbers of Protoscolices. In the infected group treated with Albendazole, the number ofProtoscolices in the GLwas very small, and the general cyst had a wrinkled appearance. As for the infected group and treated with the prepared compound, the hydatid cyst showed the presence of many erosion-like cracks in the walls of the cyst, and the absence of Protoscolices in GL.