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In this study Fusarium wilt is considered as one of the most important disease that affecte the tomato plants (Solanumlycopersicon L.), The aim of this study was identified and diagnosed the Fusarium species that cause wilt in tomato using morphological and molecular approaches. The isolates were confirmed and genetically diagnosed according to translation elongation factor TEFα1 region and recorded in the genebank DDJB. The isolation of the species F. brachygibbosum LC529919ADK7 and LC527418ADK23 for F. acutatum was performed and the pathogenicity test of the aforementioned isolates was recorded. It reached 80% to F. brachygibbosum and the isolation of F. acutatum reached 58%, as yellowing and wilting symptoms appeared after re-infection with the aforementioned isolates on healthy tomato plants, and this confirms the ability of Fusarium isolates to heterogeneity and produce strains capable of attacking the host continuously.