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The current study aims to determine the causes of Acute Flaccid Paralysis AFP cases for ages less than 15 years. Four hundred eighty stool specimens were collected during the period from January to December 2019 and received to National Polio Laboratory ( NPL\ IRAQ) that is responsible for the laboratory part of AFP surveillance project specimens collected from all Iraqi provinces.
The results showed that 65 (13.5%) sample from total samples are non –polio viruses that have specific cytopathic effects (CPE ) on cell culture in RD cells only, while 11 ( 2.3 %) samples were positive for polio virus which isolated from AFP cases, all these cases showed CPE on L20B cells only. All these samples were from patient vaccinated with the type of OPV in varying number of orally bivalent oral polio vaccine (bOPV ) dose. The results also showed that 404 (84.2%) that were (Negative) no appears CPE on both cells.
All positive Poliovirus cases 11 (16.9%) were serotyping by use rt qPCR method ,the current study confirmed that Sabin like Poliovirus serotype three (SLPV3) 7 cases ( 63.6%) from all positive Poliovirus cases followed by Sabin like Poliovirus serotype one (SLPV1 ) 3 cases ( 27.2%), while this study showed one case 1 (9%) Sabin like Poliovirus serotype one (SLPV1 ) is negative (discordant) in ITD method. The results also showed no Wild polio virus or Vaccine derived poliovirus (VDPV) isolates.