Down-Regulation of Microrna-432 in Cervical Mucus as a Pioneer Marker in Early Detection of Cervical Cancer

Abstract

Objectives:-cancer of the cervix is one of the most common cancers and a leading cause of death in women worldwide .MicroRNAs (miRNAs), non-coding RNAs of 19–25 nucleotides in length,gene expression modulation partially by pairing with the 3′ untranslated region of their messenger RNAs target.

Aim of the study:to evaluate whether the detection of miRNA-432 is a biomarker for cervical cancer and the own precursor lesions by using cervical mucus and fresh punch biopsy specimens obtained from enrolled patients by using Real -Time PCR .

Materials and Methods:- Real-Time PCR was done to demonstrate the gene expression of microRNA-432 in cervical mucus and fresh punch tissue  sample of patients with cervical cancer, precancerous , and control groups. The levels of expression of microRNA-432 were estimatedrelative to RNA messenger of Glyceraldehyde    3-phosphate dehydrogenase .

Results:-MicroRNA-432 expression level in tissue and mucus sample was significantly down-regulated in cervicalcancer in comparison to normal control,CINII and CIN III .MicroRNA-432 expression level in tissue and mucus sample was significantly down-regulated in CINII and CIN III in comparison to normal control .

Conclusion:-Cervical mucus- microRNA-432 gene expression can serve as less minimally-invasive biomarkers and standard screening tool for early diagnosis  of precancerous lesion andcancer of the cervix.