Study of The Antioxidant Activity and Inhibition Effect of Propolis Extract on Some Microorganisms
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Abstract
The study was conducted on types of spring and autumn propolis extracts and wax mixtures with propolis using two solvents, namely ethanol and diethyl ether, and their effect on the percentages of antioxidant activity. The results of the statistical analysis showed a significant effect (p<0.05) among the treatments, as spring propolis gave the highest activity when used with ethanol solvent, which reached 50.68%, followed by the mixture of wax and propolis, then the autumn propolis. However, using the solvent diethyl ether had the highest activity with the mixture of wax and propolis 74.65% at a concentration of 90 mg/ml and was less than the efficacy of Butylated Hydroxy Tolune (BHT). Whereas, the inhibitory activity of two types of propolis and a mixture of wax with propolis and between the comparison treatment (solvent) with addition rates of 0.1 ml and 0.2 ml by the agar well diffusion method was studied for four species of fungus, Penicillium sp, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger and three types of yeasts: Candida albicans Candida glabrata and Candida parapsilosis. The statistical analysis results showed an inhibitory effect with a significant difference (P˂0.05) against the studied organisms and between the types of propolis and between the size of the addition and the types of solvents used.