Molecular Detection of Eight Intsapi (S) In the Skin Pathogen Staphylococcus Aureus MRSA Clinical Strains in Iraq

Abstract

The present work highlights the tracing of eight intSapI(s) in thirty Staphylococcus aureus MRSA clinical strains isolated from skin lesions at Diwaniyah Teaching Hospital in Iraq in a previous study. A partial fragment of each intSaPI was amplified by conventional PCR using intSaPI specific primers. The amplified PCR products of the eight intSaPI(s) were 719, 539, 760, 673, 331, 472, 441, and 870 bp for intSaPI1, intSaPI2, intSaPI3, intSaPI4, intSaPI5, intSaPIn1/m1, intSaPIbov, and intSaPImw2, respectively. The frequency of occurrence of  intSaPI1, intSaPI2, intSaPI3, intSaPI4, intSaPI5, intSaPIn1/m1, intSaPIbov1, and intSaPImw2  among the thirtyMRSA clinical strains were 6.66 (n=2/30), 16.66 (n=5/30), 13.33 (n=4/30), 20 (n=6/30), 10 (n=3/30), 23.33 (n=7/30), 3.33(n=1/30), and 6.66% (n=2/30), respectively. The present data would underpin the potential of intSapI(s) in St.aureus MRSA clinical strains in pathogenesis.