Sub-minimum Inhibition Dose of Cefotaxime Diminishes Biofilm Arrangement by Staphylococcus aureus and Pseudomonas aeruginosa in vitro.

Abstract

The phenomenon of biofilm is the most important challenges for physicians. Biofilm contributes to increasing the resistance of pathogenic bacteria to antibiotics. In the current study, Staphylococcus aureus and Pseudomonas aeruginosa were isolated from infected wounds. The capacity  of these two isolates to form a biofilm in vitro was checked. A standard microtiter plate was used for this. The effect of non-inhibiting dose of cefotaxime (0.5 x MIC) on the ability of these two isolates to form biofilm was also determined. The study showed the great ability of these bacteria  to form the biofilm. The study also reported that the treatment of these two isolates with a non-inhibiting dose (0.5 x MIC) of cefotaxime that corresponding to treat S. aureus with 64.5 µg/ml and P. aeruginosa with 125 µg/ml greatly contributed to reduce the formation of biofilm these two isolates. Finaly, concluded from this study that the use of 0.5 x MIC of cefotaximereduces biofilm of S. aureus and P. aeruginosa in vitro and that finding very important in the treatment of infection with low dose of antibiotics.