Analysis of Pigment Production from Soil Pseudomonas Putida

Abstract

The environment conservation and human safety has created awareness for natural pigment sources. Microbial pigments shows better biodegrability and have numerous applications from food to cosmetics when compared to synthetic pigments. The current study aimed to screen and identify pigment producing microbial isolates especially pseudomonas using pseudomonas isolation agar from garden soil. Various time incubation were provided to optimize its maximum pigment production. During the incubation of P.putida in nutrient broth, for the first four days, there was a slight colour change in the media. The colour change was notable from the sixth day onwards. On the eighth day, it was dark green and continued on until the end of the incubation time. The validity of this experiment was demonstrated by colorimetric observation also. The pigment concentration was low (0.089±0.08) during the second day of colorimetric reading. At the time of the sixth and eighth day of colorimetric observation, the concentration of pigments was gradually increased. The concentration of pigment production was high during the tenth day of optical density observation (0.527±0.16).  The colorimetric reading in the subsequent days was more or less the same. The density of the pigments remained intact.