Isolation and Characterization of Patulin Mycotoxin from strawberry fruits
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Abstract
pencillium expansum is the most common fungi isolated from strawberry furits. The frequency and visibility of pencillium expansum is (40 and 86) respectively,on Sabouraud dextrose agar.
Patulin is extracted from the penicillin expansum that grows in the strawberry fruit. Concentration of patulin in the Strawberry fruits is (14.158-24.843) µg/ml
HPLC results show the retention time of major peak of patulin standard toxin located at 5.098 minutes, while a filter extract of p.expansum from (5.095) which is located in the same area .
The UV-VIS spectroscopy results of p.expansum and the standard patulin toxin, the major peak of standard patulin toxin is located with wavelength 276 nm and 2 absorbance; while the extract of p. expansum which is producing patulin toxin it located at a wavelength of 277 nm and 2.3 A.