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INTRODUCTION: Biomedical waste as the name suggests is the waste generated as a result of various activities and processes in Medical and Healthcare field. It is the outcome majorly because of patient care, treatment, surgical procedures, and laboratory tastings, also with researches conducted and related to healthcare. AIM: To assess the knowledge, attitude and practices of Healthcare workers towards management of biomedical waste in the hospital. RESULTS: 194 participants who consented were included in the study. Study comprising 112 nursing staff, 35 Laboratory Technicians and 47 housekeeping staff. Most of the health workers belongs to 30-40 years age group (45.88%). 60.82% participants were female workers. 34.54% participants were having experience between 6-10 years. 28.57% nursing staff, 17.14% Laboratory Technician and 6.38% Housekeeping staff had excellent knowledge regarding biomedical waste management. CONCLUSION: Most of the responses were between good to average categories so improvement of awareness regarding biomedical waste management and handling should be focused with particularly more attention on housekeeping staff. This can be achieved by regular training and awareness programs.