Anti-inflammatory Effect of Grape Seed Proanthocyanidin Extract

Abstract

The present study was carried out to evaluate the anti-inflammatory effect of grape seed proanthocyanidin extract. This study was performed by determined the   thickness of paw skin (Carrageenan Test) and differential count of WBC (Basophil, Esinophil, Neutrophil, Monocyte and Lymphocyte) in blood serum samples in mice model. Twenty eight (28) male mice were divided into four equal groups and used for anti-inflammatory effects. First group (Control) received distilled water only, second group treated with 200 mg/kg of grape seed extract (GSE), third group treated with 200 mg/kg of grape seed proanthocyanidin extract (GSPE). Fourth group treated with indomethacin (0.36 mg/kg B.W). Fifty microliters of carrageenan (1%) was injected intradermally in the paw of mice after (30) minutes following the above treatments which administrated orally. The results showed that the animals that treated with carrageenan were suffered from increasing in the inflammation by significant increasing in skin thickness and also, significant differences in the differential count of WBC (Basophil, Esinophil, Neutrophil, Monocyte and Lymphocyte) in blood serum samples. While grape seed extract (GSE), grape seed proanthocyanidin extract (GSPE) showed decreasing in the thickness of skin and differences in the counts of Basophil, Esinophil, Neutrophil, Monocyte and Lymphocyte  as compared with indomethacin. In this study the grape seed extract (GSE) have ability to reduce the inflammation that induced by carrageenan when compared with control group, the animals that treated with proanthocyanidin extract (GSPE) and the animals that treated with indomethacin.