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Hashimoto's thyroiditis (HT) is the most common organ-specific autoimmune disorder which causes destruction of the thyroid gland. The incidence of HT seems to be increasing which has been linked to excess iodine intake particularly in coastal areas. This is study is put forward to describe the potentiality of individualized homeopathic treatment of HT in female adults by observing changes in anti- TPO Ab titer in 30 patients presented with symptoms of hypothyroidism along with elevated anti- TPO Ab titer follow ups were done for 6 months in Sarada Krishna Homoeopathic Medical Collegiate Hospital, Kanniyakumari. Effectiveness of the treatment is determined by comparing the before and after treatment of Zuwelski score and anti- TPO Ab titer were statistical analyzed by using Paired ‘t’ test, the calculated value of ‘t’ for anti- TPO Ab titer is 23.80212. The value of p is < 0.00001. Thus, individualized homeopathic medicine was found effective in reducing the level of anti- TPO Ab titer in the treatment of HT among which Natrium muriaticum was prescribed in most of the cases.