Identification of Candidia Albicans associated with Angular Cheilitis by Polymerize Chain Reaction and Culture media

Abstract

Background: The candida albicans is most possible causative factor of angular cheilitis, this type of microorganism diagnosed by different laboratory tests (Culture and sugar fermentation); with polymerase chain reaction more accurate detectable tool.

Aims of the study: Laboratory detection ofcandida albicans related to angular cheilitis.

Subjects, Materials and Methods:  Isolating and identifying candida albicans by Culture media, detecting and confirming Candida albicans by Polymerase Chain Reaction technique and to distinguish Candida albicans by germ tube test, then finally; noticing Candida albicans by sugar fermentation test.

Results: Diagnosis of patients with angular cheilitis was in cooperation with oral medicine specialists as team work in the oral medicine clinic. The Sabouraud dextrose agar, HiChrome Candida differential agar, Sugar fermentation medium agar, DNA ladder marker.

Conclusions: Both cultures and polymerase chain reaction techniques are the most famous principles for growth of candida albicans