Molecular Detection of Flu A and Flu B Genes from Commensal E.Coli Isolation from School Age Children in AL-Muthannaa City

Abstract

 From the beginning of October 2019 until mid-March of 2020, 400 urine samples were obtained from school children age between of 6-12 years , males and females from 10 schools and various areas in Al-Muthannaa governorate. 10% of the total samples collected by the mean sample method, 40 urine samples for fermentation of lactose sugar appeared on the  culture media  (MacConkey,EMB), and stool samples were collected from the same people, and they were also positive, only 64 (32 urine + 32 stool) (80 %)  samples were confirmed by the vitek 2 system as E.coli , while 16 (8 urine + 8 stool) (20%) samples showed another bacteria. Polymerase chain reaction technique has been used to detect some of virulence genes Flu, and CsgA which encoding by E.coli isolates. The results demonstrated that out of total 40 isolates (20 urine + 20 stool)  there were 25 isolate (62.5%) was positive for Flu gene , The prevalence of Flu gene in commensal was significantly lower (50%) than that in recurrent higher UPEC (75%). 31 isolate (77.5 %) were positive for CsgA gene , We found a high prevalence (80%) of CsgA in UPEC isolates, while CsgA gene was detected in (75%) of the intestinal commensal isolates . In this study, describe the profile of E.coli bacteria isolated from healthy Iraqi children with no symptoms, for UTI or diarrhea by the identification of virulence genes.