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Background: Magnesium oxide nanoparticles (MgO NPs) were made using a biological method that included Leuconostoc isolate, magnesium nitrate, and NaOH as reducing agents. Methodology: This approach is both environmentally sustainable and non-toxic. In this experiment, 0.1 M (40 ml) magnesium nitrate Mg(NO₃)₂.6H₂O was introduced and held at 37ᵒC in a dark place with regular stirring at 1500 rpm. After 24 hours, the reaction mixture was dropped-wise with 2M NaOH solution to achieve a pH of 10-12. After adding the alkaline solution, a Mg (OH)₂ solution was formulated and left for 2 hours before the color changed to a brown color, then chaeacterization of MgO NPs by, UV-Visible spectroscopy, Fourier transforms infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), finally, scanning electron microscopy (FE-SEM).Results: FTIR spectra showed various functional groups in MgO NPs, which were also present in the bacterial extract. The MgO NPs were roughly with average in size, 43.88 nm, and spherical in shape when examined by SEM.The crystalline nature of MgO NPs was established using X ray diffraction (XRD) analysis. Conclusion: The current approach suggests that developing a biological process for mass-scale development of magnesium oxide nanoparticles may be possible.