Extraction and Purification of Staphylolysin Enzyme from Local Isolate of Pseudomonas Aeruginosa

Abstract

Staphylolycin is one of the important enzymes produced by Pseudomonas aeruginosa as a competitive way against other bacterial species such Staphylococcus aureus.Pseudomonas aeruginosa was isolated from a number of clinical specimens, including urine, sputum, wound, and burn swabs. On Tryptic soya agar with heat killed Staphylococcus aureus at temperature 100°C, the capacity of local isolates to produce staphylolysin enzyme was investigated. The Staphylolytic activity was extracted by cooling centrifugation and partially purified by ammonium sulphate precipitation at a saturation percentage of 80%, followed by Ion exchange chromatography using a Dowex-column (at 1.5 M NaCl) with purification folds and recovery of 2.04 fold, while SEC gave  1.51 fold .