The Detection of Ribosomal Mutations of Enterococcuscasseliflavus and Enterococcusgallinarum Isolated from Chronically Illness Patients

Abstract

Background:The enterococci are Gram-positive lactic acid bacteria that inhabit different hosts' gastrointestinal tracts.E. casseliflavus and E. gallinarum are motile, low-level vancomycin resistant and opportunistic bacteria associated with infections in immunocompromised patients

Patients and methods: first case was febrile 44-year-old man, blood samples were collected and cultured to investigate infecting bloodstream organism. Second case was 65-year-old man chronically ill with prostate disorder with urinary tract infection, urine sample was collected and cultured to investigate the microbial cause.

Results: blood and urine culture were positive for bacterial growth which characterized by amplification of 16S rRNA gene.The sequence result of 520bp ribosomal replicon revealed that local samples belong to Enterococcus casseliflavus and E.gallinarumrespectively.Sequencing results showed a total of seventeen mutations distributed in the investigated samples. Interestingly, both observed variations in both investigated samples were positioned in the same phylogenetic location in the currently generated comprehensive tree

Conclusion:The  immunocompromised people are most vulnerable to infection with  E.casseliflavus and E.gallinarum. additionally, the utilization of PCR-sequencing strategy in tow analyzed DNA samples of these local bacterial isolates have presented a confirmed identity of these strains within the Enterococuss sequences.