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Background:disinfectants and effective biosecurity measures will be critically important to control microbial diseases.Disinfectant resistance has the potential to change our way of life from compromising food security The current study aim to investigate the bacterial and resistance profile among surgical site infected patients Methodology: Eighty SSI swap from patient with age between (15-70) year, also 80 swap from hospitals environmental were collected in this study during a period of 2 months (September to October,2020). The specimens were collected aseptically. The area around the surgical wound was cleaned with 70% ethyl alcohol and the exudates was collected from the depth of the wound using two sterile cotton swabs. The specimens were processed as soon as it reached the laboratory following the standard laboratory procedures. One of the two surgical specimens, was used for gram staining and other for isolation of bacteria by culture. All specimens were cultured inoculated on Chromogenic agar UTI .The total genomic DNA were extracted using G-spinTM Genomic DNA Extraction Kit [for Bacteria] according to the manufacturer instruction (IntronBio/Korea). PCR was performed to amplify genes. Results: The results of culturing showed that Out of 80 clinically specimens 77 (96.25%) were culture positive and 3 (3.75%) were negative culture. The numbers of isolates 53 isolates .The most commonly pathogens isolated with pure cultures were Staphylococcus aureus 21 isolates (27.27 %) and Escherichia coli 14 isolates (18.18%), followed by Klebsiella pneumonia 8 isolates (10.38%) Pseudomonas aeruginosa 5 isolates (6.49%), Enterococcus fecalis 5 isolate (6.49%). While the mix culture 24(31.16).The isolation results of 80 hospitals environmental specimens was 43 (53.75%) were culture positive and 37 (46.25%) were culture negative. The numbers of isolates 39 isolates. The most commonly isolated pathogens with pure cultures were Staphylococcus aureus 10 isolates (23.25 %) and Escherichia coli 8 isolates (18.60%), followed by Pseudomonas aeruginosa 14 isolates (32.55%),Enterococcus fecalis 5 isolate (11.62) , Klebsiella pneumoniae 2 isolates (4.65%) , Mix cultures 4 (9.30 %).The genotype result of study bacterial isolate of disinfectant resistant showed that S.aureus percentage resistant of gene (qacA, qacE, SugE, mdfA, ydgF) (57.1% , 100% , 90.4% ,100% , 100% ). E.fecalis percentage resistant of gene (qacA, qacE, SugE, mdfA, ydgF) (40% , 100% , 100% ,100% , 100% ). E.coli percentage resistant of gene (qacΔE1, qacE, CepA, SugE, mdfA, ydgF) (100% , 64.2% , 71.4% ,100% ,100% , 100% ). K.penumonia percentage resistant of gene (qacΔE1, qacE, CepA ,SugE, mdfA, ydgF) (75% , 100% , 100% ,100% , 100% , 75%). P.aurogenosa percentage resistant of gene (qacΔE1, qacE,CepA , SugE, mdfA, ydgF) (100% , 100% , 100% ,100% , 100% , 100%).