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The amideligand type N2S2 have been prepared through one step. Included the reaction between one equivalent of ethylene di amine and oneequivalent of mercapto acetic acid compound. the Mn+2, Zn+2,Fe+3from there action of theligand Complexes were preparedwith metalions (1:1) ratio, the prepared compounds were characterized by (FT-IR, UV-Vis, 1HNMR) for the ligand and complexes,(C.H.N)-spectoscopies, as well as the molar conductivity and magnetic successptibility for preparedcomplexes. Thesemeasurements showsallthecomplexes have octahedral shape.and make biological study with two types of bacteria(grampositive and gramnegative).