Antimicrobial Effect of Pistacia Atlantica Kurdica as an Intra Canal Medicament in Root Canal Treatment on Enterococcus Fecalis

Abstract

Background: Pistachio Atlantica kurdica (PAK) is an aromatic resin and has many therapeutic effects such as anti bacterial, and anti fungal effects. This study was designed to investigate the antimicrobial properties of PAK resin as an intracanal medicament in failed root canal on Enterococcus fecalis in extracting teeth.

Material and Methods: Resin extracted was done by alcoholic ethanol 70% and Dimethyl sulfoxide DMSO 100% respectively. Minimal inhibition concentration identified by spectrophotometer. While the inhibition zone was determined by disc and microwell techniques. Forty-five extracted teeth randomly were divided into three groups of fourteen. They were instrumented sterilized, and then re-infected with standardized Enterococcus Faecalis. After overnight incubation, the intracanal medicament was placed inside the canal. The first group considered as a negative control, Group2 and Grou3 were treated with PAK resin and CHX 2% gel (intracanal medicament) correspondingly.

Result: The minimal inhibitor concentration of resin was 5x10^-3 mg/ml. The resin of PAK dose-dependently increased zone inhibition diameter while the colony-forming unit (CFU) of treated teeth with PAK resin was significantly reduced. However non-significant differences were found in the reduction of CFU between CHX and PAK.

Conclusion: Resin of PAK as intra-canal medicament has an excellent effect to eliminate enterococcus fecalis with no-significant differences to CHX. Minimal inhibition concentration of PAK is (0.5x10^-3 mg/ml).