Anti-gout arthritic activities of Ethanolic and Zinc oxide Nanoparticle extracts of Citrullus colocynthis- An In vitro and Insilico studies

Abstract

The aim of the present investigation is to evaluate and compare the anti gout activity of the ethanolic stem extract of Citrulluscolocynthis (ESECC) with that zinc oxide nano particle loaded ethanolic extract of Citrulluscolocynthis(ESECC ZnNP).The antioxidant activity in terms of DPPH and Hyrogen peroxide scavenging and The invitroanti-gout arthritic activity in terms of  their inhibitory potential of xanthine oxidase, protein denaturation and membrane stabilization were evaluated  for these extracts using standard protocols. In silicoanti-gout arthritic activity was examined by molecular docking using the 16 phyto ligands derived from GC Ms analysis with that of the TLR2 and TLR4  receptor proteins, in order to find  out the antagonistic nature in arresting the immunological signaling process. Among these extracts the Zinc oxide loaded nano particle of Citrulluscolocynthisexhibited better  anti gout activity in terms of inhibiting the xanthine oxidase, protein denaturation and exhibiting higher  antioxidant and membrane stabilizing potential.In the docking analysis based on the docking score and amino acid interactions three ligands namely methylvinyphenol, pentadecanoic acid and phytol has the potential to inhibit the TLR2  and TLR4 receptors there by serves as a better antagonistic ligand to suppress immunological reactions observed in the gouty arthritis. Itmaybe concluded that the Zinc oxide loaded nano particle of Citrulluscolocynthis  can be further developed into a effective drug for the management of gouty arthritis due to its antioxidant and suppressive nature of immunological reactions.