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Plants are used as a source of pharmaceutical due to presence of certain chemicals/ ingredients inside of their body and they know as medicinal plants, these are marked as a prime and effective source of primary health care in rural areas. Secondary metabolites of the plants are performing role to treat disorders and its types and concentration is variable among the plants. In solanum, steroidal glycoalkaloids (SGAs) are a family of secondary metabolites that are present in a wide range of plant species, including the Solanaceae. The goal of this study was to evaluate the relationship between different plant species based on expression of HMG1 (gene coding for hydroxy methyl glutaryl coenzyme A reductase), SGT1 (solanidine galactosyl transferase), and SGT2 (solanidine glucosyltransferase) genes in Solanum nigrum, S. lycopersicum and S. tuberosum. And determine the best source for steroidal glycoalkaloids to save time and cost in pharmaceutical industry based on Relative expression of three putative steroidal glycoalkaloid’s HMG1, SGT1, and SGT2 genes. Result indicated high level of expression of HMG1 gene in S. tuberosum, SGT1 in S. nigrum and SGT 2 in S. lycopersicum. These findings provide an important step toward understanding TGA variation in Solanum species then selecting the best source in pharmaceutical industries and drug preparation.