Investigation of Some Inflammatory Parameters in Patients with COVID-19 Diagnosed by Nucleocapsid Gene (N) Via RT-PCR

Abstract

This study aimed to estimate immune molecules CD-4 , CD-79, TNF-B and IL-2 in patients diagnosedwith COVID-19, since nucleocapsids (N) gene is an essential in viral genome, it has been used for the detection of the virus by PCR technique. A total of (100) COVID-19 seropositive patients were screened for this study to know level of TNF-B & IL-2 in serum of patient and show expression of CD4 and CD79. Results showed that serum samples were analyzed for  IL-2 & TNF-B by ELISA , showed highly significant increases (p<0.05) in serum level of COVID-19 patients as compared with healthy control groups , acute  revealed high as well as , increases in serum level of TNF-B significantly(p<0.05) ,while moderate cases moderate cases of disease express high increase in serum level of IL-2 significantly(p<0.05).Activated markers study revealed high expression of CD79 & CD4 in COVID-19  patients as compared with healthy normal groups .