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urine samples of 125 patients with urinary tract infection and 25 uninfected people as control were collected from Al-Salam Teaching hospital of Mosul city from the period from September 2020 to January 2021. The positive results of UTIs caused by microorganisms was 78 state with the ratio of 74.37%, 25.63% for females and males respectively. The highest ratio of infection was 34.63% for the age range between (15-28) years for both females and males, while the lowest ratio (7.69%) of infection for the age range between (1-14) years for both sex. Escherichia coli was formed The highest percentage (37.23%) of gram - negative bacteria that cause urinary tract infections, while the other gram -negative bacteria (Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Serratia ficaria and Shigella spp.) consist of (10.3, 8.97, 7.7, 1.28 and 1.28)% respectively, while the ratio of Gram-positive bacterial species (Staphylococcus aureus and Staphylococcus epidermidis) were (17.9 and 11.5)% respectively, finally the ratio of Candida albicans was (3.84%).The sensitivity of bacterial isolates to a group of antibiotics showed that all isolates were sensitive to the antibiotic meropenem while the highest percentage of bacterial isolates were resistant to ampicillin antibiotic.