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nanoparticles technologies are an sophisticated and utilized in numerous applications in biomedicine. The capability of metal nanoparticles as an microbial elimination which considered as alternative ways in treatment of MDR resistance bacteria. The current study discusses the novel approach in synthesis of gold nanoparticles using physical methods by laser source at 1064 nm with 500 mj and 1000 pulses. Synergism between gold nanoparticles and ceftriaxone against Klebsiella pneumonia had been appeared synergistic effect which analyzed using checkerboard technique.