Effects of Cisplatin on Glycemic Control and Lipid Profile in Male Albino Rats Role of Propolis

Abstract

Aims: This study aimed to investigate the effects of cisplatin on glycemic parameters ( fasting blood sugar FBS; glycosylated hemoglobin HbA1c), and  lipid profile (total cholesterol TC, triglyceride TG, high density lipoprotein HDL-c, and calculation of low density lipoprotein LDL-c, cardioprotective index HDL/LDL,cardiac risk ratio cholesterol/HDL), with the evaluation of any possible role for propolis in ameliorating adverse effects on these , in comparison to controls.

Materials and Methods: This experimental work takes 6 weeks and involved 60 male albino rats ,weighed  230±40g , divided into 6 groups, each of 10 members.

Group A, received 3 doses of cisplatin at 4 mg/kg intraperitoneally (I.P) at weekly intervals starting by the end of the 2^nd week, and killed by the end of the 6^th week.

Group B, received propolis  at 120 mg /day orally for 6 weeks.

Group C, received propolis 120 mg/day initially then by the end of the 2^nd week started cisplatin 4mg I.P weekly for 3 doses.

Group D, received propolis 60 mg/day for 6 weeks.

Group E, received propolis 60 mg daily for 6 weeks, starting cisplatin I.P 4 mg 3 doses by the end of the 2^nd week ,one week apart.

Group F, represent the control group, on normal saline orally for 6 weeks.

At the start the animals in all groups  were weighed ,a blood sample was taken then, during the experiment , weighed , physical activity and food intake were recorded, by the end of the experimental duration weight were recorded, a blood sample were taken .The blood samples taken before and after the experiment were for measurement of FBS, HbA1c , TC, TG, HDL-c, and calculation of  LDL-c, HDL/LDL, cholesterol/HDL.

Results:

By observation: By comparison of before and 6 weeks after the experiment, there was a significant weight reduction in the group A, with death of 2 members of this group after the 3^rd dose of cisplatin. While group B showed a significant weight gain. Clear and significant changes were recorded in parameters of lipid profile , with a insignificant effects of  CP on glycemic parameters FBG and HbA1c in group A in comparison to control group with a clear ameliorative effects of propolis on the parameters of lipid profile under study.

Conclusion: CP clearly negatively affects some of the lipid profile parameters and propolis administration clearly ameliorated the adverse effects of cisplatin on the lipid profile under study.