Isolation and Molecular Detection of Virulence Factor(Gtfb) and (Gtfc) to Streptococcus Mutans Associated with Dental Caries in Kut City of Iraq

Abstract

 Dental caries is perhaps the most prevalent chronic disease. The outcome of the disease is dental decay. The aim of this study detection of virulence factor of S. mutans glucosyltransferases (gtfB) and (gtfC). One hundred and twenty-four samples were collected from patients by taking swabs from mouth cavity. Those patients were attended at Al-Kut city, in dental specialist center and Teiba specialized medical center for the purpose of isolation and diagnosis of Streptococcus then examined under microscope and private clinics in Waist province during the period from August 2020 to December 2020. The result of PCR showed that 90(72.5%) isolates were PCR positive for gtfB gene and 34(27.4%) were PCR negative. The gtfC gene in sixty-seven isolates of Streotococcus mutans gives PCR positive results in 90(72.5%) while 34(27.4%) isolates were PCR negative.