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C.albicansis one of the most harmful fungus causing expansive infections among human. It specially has its impact in places like hospitals owing to easy transferability propertyof fungus. One major infection caused by group of candida species is Candiasis. This study aimed to identify frequency of C.albicans in GTI cases in clinical samples of Allahabad region. Out of 150 GTI samples, Candida albicans were observed to have highest frequency at 56.81%, followed by Candida tropicalisat 17.29%. Isolated C.albicanswere then characterized using ITS primers, thus sequence obtained were submitted to NCBI database. Ethanolic and aqueous extracts of Aole vera gel were assessed for antifungal activity, concluding remarkable antifungal potential (18.23±0.86 mm) againstC.albicanswhich was found to be comparable toClotrimazole (21.00±0.20mm) and much higher than the aqueous extracts. In recent years, microbes have become resistant to many drugs at very fast rate, which is issue of concern for all, therefore we emphasized on traditional medicine, like Aloe vera (L.) Burm. f., to explore more antifungal agents from them.