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The effect of inhibitory efficacy of water and alcoholic Typha domingensis pollen extracts and powder solution, as well as the effect of minimal inhibitory concentrations MIC on two types of bacterial isolates: Escherichia coli and Staphylococcus aureus, Candida albicans and Aspergillus niger pathogenic to humans. The results showed that the aqueous and alcoholic extracts and the powder solution of all concentrations (0.05, 0.1, 0.2, 0.3, 0.4, 0.5) mg. L-1 had an inhibitory effect against bacterial isolates and the greatest inhibition was at 0.5 mg. L-1 for the three extracts and for both types of bacteria, The powder solution recorded the highest inhibition diameter of Staphylococcus aureus, reaching (32.76) mm, and Escherichia coli (28.65) mm. The extracts of Typha domingensis pollen showed an inhibitory effect in all concentrations in inhibiting the growth of fungal isolates, where the powder solution recorded the largest inhibitory diameter against the growth of Candida albican and Aspergillus niger fungi at a concentration of 0.5 ml.L-1, where the diameter of theinhibition zone reached (27.97, 33.71) mm, respectively. The minimum inhibitory concentration MIC ranged between (100-400) mg.ml-1.