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Taguchi statistical design was applied and processed in the “Design expert 11 software”. ANOVA was estimated using “t-test, F-value and P-value” were used to optimize the variables affecting the antimicrobial and antioxidant activities in both coral reef fungi. Significant differences between the eighteen designed media for each activity declared the importance of optimizing growth media variables before any experiment. The high agreement between the actual and predicted values indicated the accuracy of experimental work. The F-value and P-value declared the significance of the source models in both activities by the two coral fungi, along with that the salinity was the only influential variable in producing maximum antimicrobial and antioxidant activities by the two coral fungi.The regression analysis of the model revealed law standard deviation indicating the accuracy of the designed source model. The predicted R2, adjusted R2 and adequate precision values detected an adequate signal in software system.
In case of the coral reef fungal speciesA. ocharceopetaliformis, the optimum medium deduced from the system improved the antimicrobial activity by 50% and improved the antioxidant activity by 75% compared to unoptimized media. In case of B.spectabilis, the optimized media improved the two activities by 50% each.