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Cryptosporidium spp was detected and identified in Domestic dogs in Karbala province in Iraq by conventional methods and confirmed by molecular diagnosis by using a nested polymerase chain reaction (nPCR) technique by outer primer 18s (9005bp) and inner primer 18s (830bp) were used. This was conducted during the period from beginning of December (2019) to September (2020). A total of 200 samples were collected from adult and young and from both sexes of dogs.result was record infection rate of Cryptosporidium spp in dogs 34.5 % (69/200). The higher infection rate 34.76% (44/79), was recorded in young’s dogs than adult dogs 30.25% (25/121), and the higher infection rate according to sex was found in females than males 48.27% (42/87) and 23.89% (27/113) respectively. The various infection rates were recorded during the months of the study, A higher infection rates 56% (14/25) in December (2019) and the lower infection rate 16% (4/25) infection rate in July.
The infection rates in dogs in DNA samples showed 47% (47/100) that were collected randomly from total 200 fecal samples of dogs in Karbala city. In dogs Phylogenetic analysis was done by use outer 18s (900bp) primers and outer (830bp). The result of the sequence analysis for 10 isolates randomly from the positive nested PCR samples recorded at the National Center for Biotechnology Information (NCBI) was indicate 7 isolate Cryptosporidium parvum and their accession number (MT329015.1 - MT329013.1) and 3 isolate Cryptosporidium canis and their accession number (MT329014.1, MT329016.1, and MT329018.1).