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The study aimed to evaluate the correlation among methylenetetrahydrofolate reductase gene (C677T) polymorphism and Recurrent Pregnancy Loss in Andhra Pradesh women.
Blood samples were obtained from patients with three or more repeated abortions prior to 22 weeks of pregnancy (n = 128) against control (n = 144) who have no pregnancy complications. DNA was extracted through standard Phenol-chloroform method. PCR was done for gene annealing and samples were tested for MTHFR C677T polymorphism using the sanger’s di-deoxy method. Functional significance of the observed C677T mutation was analyzed using computational tools.
The frequency of C677T mutation was CC (63%), CT (29%), TT (8%) in the patient group, and CC (79%), CT (19%), and TT (2%) in the control group (P=0.0025), which indicates significant association.
Conclusion: The outcome of the study recommends MTHFR mutation may be linked with RPL in the analyzed population.