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The current study was conducted on Entamoeaba gingivalis, where only 75 male samples were collected in the form of swabs from the periodontal pocket from the subjects visiting the periodontal branch of the Teaching Hospital of the Faculty of Dentistry of the College of Dentistry / University of Mosul during the period from 1/12/2019 to 1/12/2020. Among 59 samples of males with gum disease and 16 samples from healthy males without gum disease, the total infection rate of the amoeba parasite for males was 74%, distributed among males with gum disease, where the infection rate was 81.35% and among males without gum disease 50%. A number of biochemical tests were performed on males with gum disease and some of them had hypertension and diabetes, and included measuring the concentration of cortisol, as well as measuring the effectiveness of the α-amylase enzyme. An increase in the concentration of the hormone cotisol and a decrease in the effectiveness of α- amylase were observed in the group of males with chronic periodontitis compared with the rest of the groups, which include an affected group With parasite with damage to the support tissue, and a group infected with bleeding gums and infection with parasite, compared to the control group infected with parasite and normal, as shown To study an increase in the concentration of the hormone cortisol, as well as a decrease in the activity of the enzyme of α-amylase in the group with pressure and diabetes together compared with the rest of the groups, which include infection with the parasite with diabetes and infection with the parasite with pressure compared with the control group at a probability level of (p ≦0.005).